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System overview. (a) Cells suspended in a viscoelastic fluid are pumped through a microfluidic channel (40 μm × 30 μm) where they deform and become elongated. The chip contains an array of micro-electrodes that measures the shape of the cell along two different axes. The electrodes are connected to amplifiers and a lock-in to extract the impedance signals. The lock-in also provides a trigger signal. (b) The channel is divided into two parts: the electrical impedance sensing zone and the optical image capture zone. The two electrode configurations measure the vertical (Δ| I 1 |) and horizontal (Δ| I 2 |) dimensions of the cell. An image is focused onto a simple <t>CMOS</t> camera with a ×20 objective. (c) The trigger from the lock-in sends a pulse to a high intensity LED creating an image in the camera. (d) Definition of Electrical and Optical Deformability (ED & OD).
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System overview. (a) Cells suspended in a viscoelastic fluid are pumped through a microfluidic channel (40 μm × 30 μm) where they deform and become elongated. The chip contains an array of micro-electrodes that measures the shape of the cell along two different axes. The electrodes are connected to amplifiers and a lock-in to extract the impedance signals. The lock-in also provides a trigger signal. (b) The channel is divided into two parts: the electrical impedance sensing zone and the optical image capture zone. The two electrode configurations measure the vertical (Δ| I 1 |) and horizontal (Δ| I 2 |) dimensions of the cell. An image is focused onto a simple <t>CMOS</t> camera with a ×20 objective. (c) The trigger from the lock-in sends a pulse to a high intensity LED creating an image in the camera. (d) Definition of Electrical and Optical Deformability (ED & OD).
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System overview. (a) Cells suspended in a viscoelastic fluid are pumped through a microfluidic channel (40 μm × 30 μm) where they deform and become elongated. The chip contains an array of micro-electrodes that measures the shape of the cell along two different axes. The electrodes are connected to amplifiers and a lock-in to extract the impedance signals. The lock-in also provides a trigger signal. (b) The channel is divided into two parts: the electrical impedance sensing zone and the optical image capture zone. The two electrode configurations measure the vertical (Δ| I 1 |) and horizontal (Δ| I 2 |) dimensions of the cell. An image is focused onto a simple CMOS camera with a ×20 objective. (c) The trigger from the lock-in sends a pulse to a high intensity LED creating an image in the camera. (d) Definition of Electrical and Optical Deformability (ED & OD).

Journal: RSC Advances

Article Title: A combined electro-optical deformability micro-cytometer †

doi: 10.1039/d4ra04800h

Figure Lengend Snippet: System overview. (a) Cells suspended in a viscoelastic fluid are pumped through a microfluidic channel (40 μm × 30 μm) where they deform and become elongated. The chip contains an array of micro-electrodes that measures the shape of the cell along two different axes. The electrodes are connected to amplifiers and a lock-in to extract the impedance signals. The lock-in also provides a trigger signal. (b) The channel is divided into two parts: the electrical impedance sensing zone and the optical image capture zone. The two electrode configurations measure the vertical (Δ| I 1 |) and horizontal (Δ| I 2 |) dimensions of the cell. An image is focused onto a simple CMOS camera with a ×20 objective. (c) The trigger from the lock-in sends a pulse to a high intensity LED creating an image in the camera. (d) Definition of Electrical and Optical Deformability (ED & OD).

Article Snippet: Synchronised optical images of deformed cells are captured by triggering a high-speed LED which projects an image onto a low-cost CMOS camera (MQ003CG-CM, XIMEA).

Techniques: